![]() ![]() However, there are no data on agouti testicular tissues. Thus far, the development of protocols for the cryopreservation and in vitro culture of ovarian tissues has been reported. Various efforts have been conducted to optimize assisted reproductive techniques and the preservation of agouti germplasms. Thus, it is an excellent experimental model for the conservation of endangered hystricognath rodents, such as the D. Among agoutis, the breeding of the red-rumped agouti ( Dasyprocta leporina) is easy because it can adapt to captivity. These animals are important for the ecological balance, acting as seed dispersers and forming part of the food chain. The genus Dasyprocta, a wild hystricognath rodent, is composed of 13 different species of agoutis found throughout the Neotropical Americas. Overall, the use of SSV with DMSO + EG was the best protocol for the preservation of testicular tissues from adult red-rumped agoutis. Adequate preservation of the proliferative potential of spermatogonia, Leydig cells, and Sertoli cells was obtained using SSV with DMSO + EG. Regarding mitochondrial activity, DMSO + EG/SSV or CV, and DMSO + EG/CV were similar to the EG/SF group, which was the best group that provided values similar to fresh control groups. Across the different cryopreservation approaches, as well as cryoprotectant combinations, cell viability was comparable. Testicular morphology was better using SSV with a combination of DMSO and EG. Morphology, viability, mitochondrial activity, and proliferative potential were assessed in fresh and frozen tissue samples. Each method used dimethyl sulfoxide (DMSO), ethylene glycol (EG), or a DMSO + EG combination. Tissue biopsies (3.0 mm 3) from five individuals were allocated to different experimental groups: control (non-cryopreserved) slow freezing (SF), solid-surface vitrification (SSV), and conventional vitrification (CV). We concluded that structural characteristics are similar to other mammals, although the morphology of agouti's palate differs in the amount and disposition of palatine ridges, and the conical CTC's morphology.Ĭonnective tissue core hard palate morphology palatine ridges soft palate.This study measured the effects of different freezing techniques and permeating cryoprotectants on the preservation of testicular tissues from adult red-rumped agoutis. The structural and ultrastructural characteristics enable stability of the hard palate and fixation to the soft palate sides, while the soft palate center has greater mobility thus assisting in food swallowing. Near the CTCs were nerve fibers in the hard palate, and the soft palate had muscular tissue below the gland clusters. Epithelial removal revealed CTCs with a conical shape with high density in the hard palate and the sides of the soft palate. Stratified squamous keratinized epithelium with projections of lamina propria and soft palate had gland clusters. ![]() We found nine palatine ridges in the diastema and hard palate, and a smooth surface in the soft palate. ![]() This research aimed to describe structural and ultrastructural characteristics of the palatine epithelium and the connective tissue cores (CTCs) of ten red-rumped agoutis (Dasyprocta leporina-Linnaeus, 1758) using macroscopic, light microscopy, scanning electron microscopy, and transmission electron microscopy. The palate is a fundamental region in food swallowing and presents different adaptations in species.
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